Question

Which technique of microscopy does not involve use of stains?

• A. Transmission electron microscopy
• B. Phase contrast microscopy
• C. Scanning probe microscopy
• D. None of the above

Answer 1

The correct option is B Phase contrast microscopy

Phase contrast microscopy is an optical microscopy illumination technique in which small phase shifts in the light passing through a transparent specimen are converted into contrast changes in the image. The use of phase contrast does not require staining to view the slide. This microscope technique made it possible to study the cell cycle in live cells. Therefore, the correct answer is option B.