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Question

Which theory explains ATP synthesis in chloroplasts and mitochondria?


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Solution

Chemiosmotic hypothesis

Chemiosmotic hypothesis was given by a British biochemist by the name of Peter Dennis Mitchell. This hypothesis provides explanation for the biological process of synthesising ATP by ATP synthase.

During light reaction, photolysis of water generates protons which accumulate in the lumen (membrane of thylakoids).Movement of electrons through photosystems and reduction of NADP+ by NADP reductase removes H+ ions from stroma.

Chemiosmotic hypothesis

Thus, a proton gradient is generated due to accumulation of protons in the lumen and their decrease in the stroma.

ATP synthesis is a result of the breakdown of this proton gradient during which protons move from lumen to stroma through ATP ase situated in the membrane.

Thus, ATP synthesis is a chemiosmotic phenomenon.

Chemiosmotic hypothesis

In mitochondria also the NADH and FADH2 formed from glycolysis and kreb cycle transfer their electrons and protons to the ETS.

As a result, proton gradient is developed which is the driving force for ATP synthesis.

Final answer

(D) Chemiosmotic theory of Mitchell.


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