Question

Which type of restriction enzymes are used in recombinant DNA technology?

• A. Type-I
• B. Type-II
• C. Type-III
• D. All of the above

Answer 1

The correct option is B Type-II

Type II restriction enzymes are heavily used in recombinant DNA techniques. Type II enzymes consist of single, separate proteins for restriction and modification. One enzyme recognizes and cuts DNA, the other enzyme recognizes and methylates the DNA. Type II restriction enzymes cleave the DNA sequence at the same site at which they recognize it.