Question

Which vector can hold the largest size fragment of DNA?

Answer 1

Yeast artificial chromosomes (YAC) :

1. It is an artificially constructed vectors to carry larger gene sequences.
2. It is the largest vectors that are created with the help of yeast DNA.
3. They are genetically engineered to hold more than one million base pairs.
4. It can undergo replication and has the capability to insert foreign DNA sequences.
5. These are useful for the physical mapping of complex genomes, chromosome walking and for the cloning of large genes.