Question

White blood cells are cultured on a glucose rich nutrient media and a toxin. Which of the following will be true for cells growing at a same rate on a media without toxin?

• A. ATP production will remain the same.
• B. Glucose consumption will increase.
• C. Oxygen consumption will increase.
• D. Production will increase.
• E. None of the above.

Answer 1

Answer: (B)

Glucose consumption will increase.

A nutrient media contains all the factors which are responsible for in vitro growth, development, and preservation of different cells. Nutrient media is specific to the living organism as needs for survival varies. WBC when cultured on a glucose-rich nutrient media without toxin, the cell starts to grow by utilizing the available glucose. In the initial stages, the glucose is utilized at a slower pace but it rapidly increases as the cell enters the log phase. Glucose acts as an initiator which activates the structural genes to start the metabolization of glucose.

Hence the correct option is B.