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Question
Who developed the technique of nurse tissue to show cellular totipotency
Who developed the technique of nurse tissue to show cellular totipotency
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Solution
The correct option is C Muir
Totipotency is the genetic potential of a plant cell to produce an entire plant. A single cell can produce all cell types and organises them into an entire organism.The basis of tissue culture is to grow a large number of cells in a sterile controlled environment. The plant cells are allowed to grow in culture medium containing mineral nutrient, vitamins and hormones to encourage cell division and growth. As a result cells in culture will produce an unorganised proliferative mass of cells which is known as callus tissue.Plant cells can be cultured from isolated single plant cell on the top of the actively growing callus ( nurse tissue ) separated by a piece of filter paper. The filter paper serves to prevent tissue union but allows the flow of essential nutrients from the nurse to the isolated cells. The technique has been developed by Muir e.t. al in 1954. The single cell is isolated from, suspension cultures or friable callus with the help of a micropipette, or from the intact plant tissue ( root, stem, leaf etc. )Single cell culture is important for cell cloning, crop improvement programmes, the study of biotransformation etc.Thus, the correct answer is option C.
Totipotency is the genetic potential of a plant cell to produce an entire plant. A single cell can produce all cell types and organises them into an entire organism.
The basis of tissue culture is to grow a large number of cells in a sterile controlled environment. The plant cells are allowed to grow in culture medium containing mineral nutrient, vitamins and hormones to encourage cell division and growth. As a result cells in culture will produce an unorganised proliferative mass of cells which is known as callus tissue.
Plant cells can be cultured from isolated single plant cell on the top of the actively growing callus ( nurse tissue ) separated by a piece of filter paper. The filter paper serves to prevent tissue union but allows the flow of essential nutrients from the nurse to the isolated cells. The technique has been developed by Muir e.t. al in 1954. The single cell is isolated from, suspension cultures or friable callus with the help of a micropipette, or from the intact plant tissue ( root, stem, leaf etc. )
Single cell culture is important for cell cloning, crop improvement programmes, the study of biotransformation etc.
Thus, the correct answer is option C.
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