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Question

Why are antibiotic resistance genes used as selectable markers in E coli?


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Solution

Antibiotic resistance: When bacteria, fungus, and other microorganisms learn to resist the medications meant to kill them, this is known as antimicrobial resistance.
Selectable markers

  1. A gene put into a cell, particularly a bacterium or cells in culture, that provides a trait suited for artificial selection is known as a selectable marker.
  2. Since E. coli does not natively contain antibiotic resistance genes, these genes are employed as selectable markers in this organism.
  3. This component is necessary for the plasmid to remain in the cell.
  4. The plasmid is now valuable to the cell because it contains the selection marker.
  5. Only cells that have plasmids with the proper selectable marker can survive under the selection circumstances.

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