Question

Why are sticky ends better than blunt?

Answer 1

DNA ligation:

1. DNA is cut by the restriction enzyme that leaves the DNA with two preferred properties i.e. sticky ends and blunt ends.
2. A straight cut of restriction enzymes gives blunt ends, where both strands end with base pair.
3. It is also known as non-cohesive strands.
4. On the other side, sticky ends are cohesive strands that have either 5’- or 3’- strands, which are also known as overhangs.
5. Sticky ends are mostly used for cloning technology where a DNA ligase is used to join two DNA fragments into one.
6. Sticky ends facilitate ligation by DNA ligase by forming hydrogen bonds between complementary bases of the other strand.
7. Thus, the sticky ends are better than the blunt ends.