Question

Why are Type 2 restriction enzymes used for cloning?

Answer 1

Type 2 restriction enzymes:

1. Restriction enzymes are the enzymes produced by certain bacteria that have the property of cleaving DNA molecule at or near specific base sequences.
2. Type 2 restriction enzymes are used for many molecular biology functions such as gene cloning and DNA fragmentation and analysis.
3. These enzymes cut the DNA at fixed positions with respect to their recognition sequence.
4. Thus, it is often used for the cloning process.