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Question

Why do restriction enzymes not cut their own DNA?


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Solution

Restriction enzymes:

  1. Restriction enzymes are endonucleases that cleave DNA molecules at specific recognition sites.
  2. There are four types of restriction enzymes that are employed and they differ in structure and specificity.
  3. When it recognizes the specific site of interest, it wraps around the DNA and introduces breaks in both strands.
  4. These enzymes don't act on their own DNA as their DNA molecules lack the recognition sequences.
  5. In addition, the recognition sequences on their own DNA are highly methylated and thus are unrecognizable by the enzymes.

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