Why does bright red colour blood turn dark once circulation begins?
O2 is replaced by CO2
The oxygen molecules that the blood carries reacts with the iron compounds in the haemoglobin molecule, giving blood its characteristic red colour. As blood circulates, it delivers essential nutrients and oxygen to all organs and cells in the body. As circulation progresses, the overall O2 concentration in the blood decreases and finally it is replaced completely by CO2, thereby making it darker.