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Question
Why chilled ethanol is used during the isolation of the desired gene?
Why chilled ethanol is used during the isolation of the desired gene?
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Solution
Chilled ethanol is used because:
- Chilled ethanol has a lower polarity than water.
- The screening charges imposed by water are disrupted when it is added to the solution.
- Phosphate's electrical attraction to any positive ions (Na+) in solution becomes strong enough to create a stable ionic connection and DNA precipitates.
- Ethanol precipitation is a common method for purifying and concentrating nucleic acids.
- DNA spooling is the term for this process.
Chilled ethanol is used because:
- Chilled ethanol has a lower polarity than water.
- The screening charges imposed by water are disrupted when it is added to the solution.
- Phosphate's electrical attraction to any positive ions (Na+) in solution becomes strong enough to create a stable ionic connection and DNA precipitates.
- Ethanol precipitation is a common method for purifying and concentrating nucleic acids.
- DNA spooling is the term for this process.
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