Question

Why is it important that the pieces of DNA are cut with the same restriction enzymes?

Answer 1

Restriction enzymes:

1. The same restriction enzyme must be employed because they cut at specified sequences and generate fragments with identical complementary sticky ends, which enable bonds to form between them. Certain restriction sites that work well together can be employed.
2. Certain bacteria manufacture enzymes known as restriction enzymes that have the ability to cleave DNA molecules at or close to particular base sequences.
3. When necessary, a restriction chemical uses shape-to-shape coordination. It folds over the DNA and breaks the two strands of the DNA particle when it encounters a DNA cluster whose shape matches a portion of the compound, known as the acknowledgment site.
4. They are able to identify and connect with certain DNA successions that are known to be restriction sites. A single restriction chemical can only recall one or very few restriction locations. As it locates its intended successor, a restriction catalyst produces a twice-abandoned incision in the DNA molecule.
5. The cut occurs in a precise and predictable way, usually near or at the point of the constraint.