why we are found that the activation opf nuclotide in monophosphate to triphosphate is necessary
Because the nucleotide A will only pair with T ,and G only with C.
Deoxyribonucleotides or deoxyribonucleoside monophosphates occur freely inside the nucleoplasm. They are of four types— deAMP (deoxyadenosine monophosphate), deGMP (deoxyguanosine monophosphate), deCMP (deoxycytidine monophosphate) and deTMP (deoxythymidine monophosphate). They are first phos- phorylated and changed to active forms which have three phosphate residues instead of one. Enzymes phosphorylase is required along with energy.
The phosphorylated nucleotides are deATP (deoxyadenosine triphosphate), deGTP (deoxyguanosine triphosphate), deCTP (deoxycytidine triphosphate) and deTTP (deoxythymidine triphosphate). These triphosphates of bases serve dual purpose. They act as substrate as well as provide energy for polymerisation of nucleotides.
Deoxyribonucleotides or deoxyribonucleoside monophosphates occur freely inside the nucleoplasm. They are of four types— deAMP (deoxyadenosine monophosphate), deGMP (deoxyguanosine monophosphate), deCMP (deoxycytidine monophosphate) and deTMP (deoxythymidine monophosphate). They are first phos- phorylated and changed to active forms which have three phosphate residues instead of one. Enzymes phosphorylase is required along with energy.
The phosphorylated nucleotides are deATP (deoxyadenosine triphosphate), deGTP (deoxyguanosine triphosphate), deCTP (deoxycytidine triphosphate) and deTTP (deoxythymidine triphosphate). These triphosphates of bases serve dual purpose. They act as substrate as well as provide energy for polymerisation of nucleotides.
