Question

With the help of a neat and labelled diagram describe steps in recombinant DNA technology.

Answer 1

The basic steps involved in the process of DNA technology are as follows.
$\left(i\right)$ The genomic DNA is isolated from a donor.
$\left(ii\right)$ Using restriction enzymes such as endonucleases the DNA is fragmented. Endonuclease enzymes are known as molecular scissors as they produce nick in the DNA fragment at the desired place.
$\left(iii\right)$ The fragments were taken out are then screened for the desired gene.
$\left(iv\right)$ Fragments of DNA with the desired gene are inserted into a cloning vector. Cloning vector can be a plasmid, cosmid or even a phage DNA. This insertion makes recombinant DNA or DNA (also known as chimeric DNA).
$\left(v\right)$ The recombinant vector is introduced into a competent host cell.
$\left(vi\right)$ These cells are later cultured to obtain multiple copies or clones of the desired fragment of DNA.
$\left(vii\right)$ These copies are used to transform suitable host cells which express the desired gene.