Question

write an activity to show the tissue culture at home

Answer 1

These steps will make 1 L of growth medium, which is enough to prepare about 65 growing tubes.

1. Dissolve the MS mixture in about 800 ml of distilled water. Stir the water continuously while adding the salt mixture. Add 30 g sugar and stir to dissolve. Adjust pH to 5.8 using 1M NaOH or 1M HCl as necessary while gently stirring. Add distilled water to make the total volume up to 1 liter.
   
2. Weigh out 8 grams of agar and add it to the MS solution. Heat the solution gently while stirring until all the agar has dissolved.
   
3. Pour the still warm medium into the polycarbonate tubes to a depth of about 4 cm which will use about 15 ml of medium per tube.
   
4. Place the tubes (with lids on the tubes but not tightened) in a pressure cooker and sterilize for 20 minutes. Allow the pressure cooker to cool, then remove the tubes and tighten the lids. Alternatively, the tubes can be placed in boiling water for 30 minutes, but make sure that none of the water is able to enter the tubes