Question

Write the condition required for chromatography.

Answer 1

Chromatography is the ability to separate molecules using partitioning characteristics of molecule works on the principle of difference in movement of different molecules of a mixture in a two different phases such as stationary phase versus a mobile phase. Once a molecule is separated from the mixture, it can be isolated and quantified.

Condition of use are-

The presence of two different phases
1. Mobile phase like water , ethanol solvent etc
2. Stationary phase like paper made of cellulose over which molecules of mobile phase travels.

Except these condition varies with different type of Chromatography