Question

Write the formula for limit of resolution of microscope and explain the symbols used.

Answer 1

Limit of resolution is given by,

Limit of resolution $=d=\frac{0.61\lambda }{NA}=\frac{0.61\lambda }{\mu sin\alpha }$

where $NA=$Numerical Aperture of the microscope,

$\mu =$ Refractive index of the medium,

$\alpha =$ Half angle with the optical axis,

$\lambda =$ Wavelength of light used.