Question

Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.

Answer 1

Below are the steps to be undertaken to obtain a foreign-gene-product:

Step 1 : On insertion of a piece of DNA into cloning vector and tranfer into a bacterial plant or animal cell, the alien DNA gets multiplied.

Step 2 : After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein, one has to consider producing it on a large scale.

Step 3 : The Cultures may be used for extracting the desired protein and then purifying it by using different separation technique.

Step 4 :The cells can also be multiplied in a continous culture system. The used medium is drained out from one side, while fresh medium is added from the other to maintain the cells in their physiologically most active log exponential phase.