You are required to draw blood from a patient and to keep it in a test tube for analysis of blood corpuscles and plasma. You are also produced with the following four types of test tubes. Which of them will you not use for the purpose?
A
Test tube containing calcium bicarbonate
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B
Chilled test tube
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C
Test tube containing heparin
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D
Test tube containing sodium oxalate
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Solution
The correct option is A Test tube containing calcium bicarbonate
Clotting of collected blood can be prevented by :
Coating test tubes with silicon (which produces non-wettable surface similar in its smoothness to the endothelial lining of blood vessels)
Adding chelating agents (includes trisodium citrate, sodium oxalate, and sodium EDTA) which remove calcium which is important for blood coagulation, and prevent blood clotting. Hence test tube containing calcium carbonate cannot be used.
Adding heparin, the most powerful anticoagulant which acts indirectly by activating plasma antithrombin III.
Heparin is effective both in vivo and in vitro. Hence, the correct answer is A.