How do you calculate the dilution factor? Give me an example

To make a dilute solution without calculating concentrations, you can rely on a derivation of the above formula:
(Final Volume / Solute Volume) = Dilution Factor


Make 300 μL of a 1:250 dilution

Formula: Final Volume / Solute Volume = DF

Plug values in: (300 μL) / Solute Volume = 250

Rearrange: Solute Volume = 300 μL / 250 = 1.2 μL


Place 1.2 μL of the stock solution into 300 μL – 1.2 μL = 298.8 μL diluent

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