Question

Sanger's reagent is used for the identification of:

• A. Side chain of amino acids
• B. N-terminal of the peptide chain
• C. C-terminal of the peptide chain
• D. molecular weight of the peptide chain

Answer 1

The correct option is B

N-terminal of the peptide chain

Explanation:

Sanger's reagent:

• Sanger’s reagent is known as 1-fluoro-2,4-dinitrobenzene or dinitrofluorobenzene.
• It is abbreviated as DNBF.
• It is named after the scientist Frederick Sanger who first used it to detect free amino acids in insulin.
• Sanger’s reagent can be helpful for the sequencing of proteins.
• Sanger’s reagent is used for determining the N-terminal amino acid in polypeptide chains, in particular insulin.
• Sanger’s reagent reacts with amino groups in amino acids to produce dinitrophenyl amino acids.
• These dinitrophenyl amino acids are stable under acid hydrolysis conditions that break peptide bonds.

Therefore. option B is correct.