Cross between two parents, considering alternative traits of two different characters.
For example - Cross between wrinkled-green seeds (rryy) with round-yellow seeds (RRYY).
The phenotypic ratio in F2 generation of dihybrid cross is - 9:3:3:1
Law of Independent Assortment:
It is based on a dihybrid cross.
The law states that ‘when two pairs of traits are combined in a hybrid, segregation of one pair of characters is independent of the other pair of characters.'
Each pair of contrasting characters behaves independently and bears no association with a particular character.
Because of such an independent assortment of characters, new combinations of characters are produced in the offspring.