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Question

Does EcoRI leave blunt or sticky ends?


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Solution

Sticky ends:

  1. Sticky ends can easily bond with complementary DNA fragments.
  2. Sticky ends are produced when a restriction enzyme cuts the DNA molecule and produces overhangs.
  3. EcoRIleaves sticky ends and produces AATT overhangs at 5'.
  4. However, in blunt ends overhangs are not produced.

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