RNA Primase

Q. The enzyme DNA dependent RNA polymerase and DNA dependent DNA polymerase catalyse the polymerisation reaction in ___(i)_____ and ___(ii)_____ direction, respectively.

1. i- 5'→3', ii- 3'→5'
2. i- 5'→3', ii- 5'→3'
3. i- 3'→5', ii- 5'→3'
4. both a and c

Q.

What is Severo Ochoa enzyme and what is its function?

Q.

What is the role of RNA polymerase III in the process of transcription in eukaryotes?

Q.

What Is the purpose of Replication Fork?

Q.

What is the function of DNA polymerase I during DNA replication?

Q.

What is replication and where does it occur?

Q.

What is the difference between DNA polymerase and DNA ligase?

Q.

Does EcoRI leave blunt or sticky ends?

Q.

During transcription in eukaryotes, RNA polymerase II synthesizes

1. precursors of tRNA and snRNA

2. precursors of mRNA, snRNA and microRNA

3. precursors of rRNA

4. all of the above

Q. Which of the following statement does not hold true for restriction enzymes?

1. It is endonuclease
   
2. It recognizes palindromic nucleotide sequences
   
3. It is isolated from bacteriophages and bacteria
   
4. Different restriction enzymes produce different kinds of sticky or blunt ends depending upon their recognition sequence
   

Q.

Is RNA polymerase found in prokaryotes?

Q.

What can modify DNA molecules?

Q. The process of multiplication of DNA from DNA is known as

1. Duplication
2. Transcription
3. Translation.
4. Replication

Q. Which of the following figures correctly represents the replication fork formed during DNA replication?

1. 
2. 
3. 
4. 

Q. A template strand in a bacterial DNA has the given base sequence 5' – GGTTTAACGA – 3'
What would be the RNA sequence transcribed from this template DNA?

1. 3' CCAAATTGCT 5'
2. 5' UCGUUAAACC 3'
3. 5' CCAAATTGCT 3'
4. 3' UCGUUAAACC 3'

Q. Define DNA.

Q. Why is RNA primase important for DNA replication?

1. A primer is always made of RNA
2. None of the above
3. DNA polymerase cannot start replication from a free 5' end
4. RNA primer added to DNA strand gives stability during replication

Q. Restriction endonucleases are called so as they

1. synthesize DNA
2. restrict nuclear activity
3. cleave DNA into fragments at or near restriction sites
4. break DNA at random

Q. The given flowchart shows central dogma reverse.

Enzymes used in processes A, B and C are respectively

1. $\begin{array}{ccc}A& B& C\\ DNAdependent& RNAdependent& CDNAdependent\\ DNApolymerase& DNApolymerase& RNApolymerase\end{array}$
2. $\begin{array}{ccc}A& B& C\\ DNAdependent& DNAdependent& RNAdependent\\ DNApolymerase& RNApolymerase& DNApolymerase\end{array}$
3. $\begin{array}{ccc}A& B& C\\ DNAdependent& DNAdependent& RNAdependent\\ RNApolymerase& DNApolymerase& DNApolymerase\end{array}$
4. $\begin{array}{ccc}A& B& C\\ RNAdependent& DNAdependent& RNAdependent\\ DNApolymerase& RNApolymerase& RNApolymerase\end{array}$

Q. Match List I(Enzyme) with List II(Function) and select the correct answer using the codes given the lists.

List-I	List-II
a. DNA Ligase	$1$. Catalyses the covalent union of segments of an interrupted sugarphosphate strand in double-stranded DNA
b. DNA polymerase	$2$. End nuclease that digests either single stranded or double stranded DNA
c. DNase I	$3$. Produces single or double stranded breaks in DNA during replication to release tension brought about by the separation of double strands
d. DNA topoisomerase	$4$. Catalyses the formation of DNA worn deoxyribonucleotides using one strand of DNA as template

1. A-$1$, B-$4$, C-$3$, D-$2$
2. A-$4$, B-$1$, C-$2$, D-$3$
3. A-$1$, B-$4$, C-$2$, D-$3$
4. A-$4$, B-$1$, C-$3$, D-$2$

Q. During DNA replication, Okazaki fragments are used to elongate.

1. The lagging strand away from the replication fork
2. The leading strand towards replication fork
3. The lagging strand towards replication fork
4. The leading strand away from replication fork

Q. The type of RNA polymerase controlling DNA replication are

1. 2
2. 4
3. 3
4. Only one type

Q. Choose the correct answer from the alternatives given :
The enzyme DNA dependent RNA polymerase catalyses the polymerisation reaction in ________ direction.

1. Only 5 $\to$ 3
2. Both (a) and (b)
3. Only 3 $\to$ 5
4. None of these

Q. Why is RNA primase important for DNA replication?

1. DNA polymerase cannot start replication from a free 5' end
2. RNA primer added to DNA strand gives stability during replication
3. A primer is always made of RNA
4. None of the above

Q. Which enzyme polymerizes $RNA$ with defined sequences in a template independent manner?

Q. How many types of RNA polymerases are operative in eukaryotes?

1. Two
2. Four
3. Three
4. One

Q. Which enzyme polymerizes RNA with defined sequence in a template independent manner?

1. Peptidyl transferase
2. RNA Polymerase
3. Reverse transcription
4. Polynucleotide phosphorylase

Q. RNA primer is removed by

1. DNAP-I
2. DNAP-II
3. DNAP-III
4. Primase

Q. why replication bubbles are formed during DNA replication ?

Q. Write the function of RNA polymerase II.