Question

How can organelles be separated from cell homogenate?

Answer 1

Cell organelles:

1. The subcellular structures that perform the function of keeping the cell in working condition are referred to as cell organelles.
2. Each cell organelle is specialized to perform different functions thus, keeping the cell healthy.
3. Cellular organelles are composed of molecules like lipids, carbohydrates, proteins, and nucleic acids which include DNA and RNA, etc.

Separation of cell organelles:

1. The process of differential centrifugation is implied to separate the cell organelles from the cell homogenate.
2. Using the centrifugal force, the particles are allowed to sediment in the suspension, this is the basic principle behind this process.
3. When the accelerating force has been applied the particles present in the centrifuged sediment at different rates.
4. The rate of sedimentation is determined by the size, density, and shape of the particles.