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Standard X
Biology
Applications of Biotechnology
What is PCR i...
Question
What is PCR involved in?
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Solution
PCR:
Polymerase chain reaction or PCR is an amplification technique.
It has the ability to amplify small samples of DNA at a large scale.
PCR is involved in many research labs, forensics, genetic testing, and also in diagnostic fields.
PCR is a process of 20-40 repeat thermal changes called thermal cycles.
Each cycle consists of three different temperatures.
Steps in PCR:
Denaturation:
At temperature between 94°-95°C, denaturation of DNA occur.
Parent DNA breaks and forms two strands due to the application of high temperature.
Annealing:
At temperatures between 50°-56° C, DNA primers bind to separated stands.
Extending:
At a temperature of 72° C, Taq polymerase bind to primer and forms double-stranded DNA from a single strand.
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