Question

What triggers denaturation?

Answer 1

Denaturation:

1. A process by which DNA and proteins denature into their simpler form is known as denaturation.
2. Changes in temperature, pH, and solute concentration lead to the denaturation of proteins and DNA.

Denaturation of DNA :

1. The process by which double-stranded DNA converts into two single strands when subjected to high temperature, and high pH.
2. When the denatured DNA is subjected to low temperature and low pH, the two single strands of DNA rejoin to form double-stranded DNA is called renaturation of DNA

Denaturation of proteins :

1. The process by which the highly folded structure of protein converts into the primary structure when exposed to high temperature, high pH, and denaturing agents is known as denaturation.
2. The exposure to denaturing agents breaks down the bonds by which protein molecules were linked.
3. The denatured protein losses its functionality.
4. When the denatured protein is subjected to low temperature and low pH, the protein rejoins to form its native form and regain its functionality is termed renaturation of proteins.