Question

Which of the following sequences is recognized by the restriction enzyme BamHI?

• A. 5' - GAATC - 3'3' - CTAAG - 5'
• B. 5' - AAGCTA - 3'3' - TTCGAT - 5'
• C. 5' - GGATCC - 3'3' - CCTAGG - 5'
• D. 5' - CCCAAT - 3'3' - GGGTTA - 5'

Answer 1

The correct option is C

5' - GGATCC - 3'

3' - CCTAGG - 5'

The correct option is C.

Explanation for the correct option:

1. BamHI is a type II restriction endonuclease that can recognize short (6 bp) DNA sequences and cleave them precisely at a target spot.
2. BamHI is also a type II restriction enzyme, isolated from Bacillus amyloliquefaciens.
3. The cut is made at the G-G base pairs, generating sticky ends, and the recognition site for this is GGATCC (6 base pairs).
4. Between G and G must be the recognition point.
5. BamH1 produces sticky ends 5'GATCC 3' by cutting both strands shortly after the first guanine at the 5'end.

Explanation for the incorrect options:

Option A:

1. One of the most widely used type II restriction endonucleases, EcoR1, has this recognition sequence.

Option B:

1. It serves as the RE HindIII's location.
2. Haemophilus influenza has been the source of this isolate.
3. Similar to BamH1 and EcoR1, this results in sticky ends.

Option D:

1. The location of limitation for a blunt-end endonuclease. In the six nucleotide recognition sequence, the enzyme makes a cut at the third base from the ends.

Final answer: The sequences recognized by the restriction enzyme BamHI are 5’GGATCC 3’
3’CCTAGG 5’