Gene Silencing

Q.

RNA interference which is employed in making tobacco plant resistant to Meloidegyne incognita is essentially involved in preventing the process of

1. translation of mRNA

2. transcription

3. replication of DNA

4. splicing of hnRNA

Q. Reverse transcriptase is the enzyme which makes

1. RNA from DNA template
2. RNA from RNA template
3. DNA from RNA template
4. DNA from DNA template

Q. In pest resistant plants that use gene silencing method, host plant generated dsRNA triggers protection against

1. Rhizobium infestation
2. Nematode infestation
3. Bacterial infestation
4. Nostoc infestation

Q.

How is RNA used in biotechnology?

Q. RNAi helps in producing pest resistant plants through the formation of

1. dsDNA
2. ssDNA
3. ssRNA
4. dsRNA

Q.

RNA interference is:

1. Inhibiting activity of a gene through production of sense and antisense.

2. Mutation in mRNA.

3. Inserting RNA molecule in between DNA.

4. Formation DNA from RNA.

Q.

Given below are a few statements. Choose the correct answer in reference to these statements

Statement 1: RNAi technique is used for gene knockout

Statement 2: Gene silencing or gene knockdown can be achieved by RNAi technique

Statement 3: siRNA suppresses gene expression converting mRNA into cDNA

Statement 4: siRNA suppresses gene expression by degrading mRNA

1. Statement 1 is incorrect while statement 3 is correct
2. Statement 2 and 4 are correct
3. Statement 3 and 4 are incorrect
4. Statement 1 and 3 are correct

Q. Pilus is the appendage required for bacterial ________.

1. Cell division
2. Conjugation
3. Movement
4. None of the above

Q.

Enzyme polygalacturonase is responsible for

1. Vegetable softening

2. Fruit softening

3. Tomato hardening

4. Sugarcane rotting

Q.

RNA interference which is employed in making tobacco plant resistant to Meloidegyne incognita is essentially involved in preventing the process of

1. translation of mRNA

2. transcription

3. replication of DNA

4. splicing of hnRNA

Q. RNAi is the important cellular defence in

1. Prokaryotes
2. Eukaryotes
3. All living organism
4. Viruses

Q.

Silencing of mRNA has been used in producing transgenic plants resistant to:

1. Bollworms

2. Nematodes

3. White rusts

4. Bacterial blights

Q.

RNA interference is:

1. Mutation in mRNA.

2. Inserting RNA molecule in between DNA.

3. Inhibiting activity of a gene through production of sense and antisense.

4. Formation DNA from RNA.

Q. Which famous biologist attenuated. the germs of anthrax disease by heating them and inoculated sheep with them, thus making the sheep resistant to anthrax?

1. Lazaro Spailanzani
2. Francesco Redi
3. Louis Pasteur
4. Joseph Lister