Restriction Enzyme

Q.

Why is pBR322 a useful vector?

Q. The usual source of restriction endonucleases used in gene cloning is from

1. fungi
2. bacteria
3. plants
4. viruses

Q. What is Structure of DNA ? How many types of DNA ? What is process or condition to change of DNA to RNA ?

Q. Which of the following is not a tool in genetic engineering?

1. Exonuclease
2. Restriction endonucleases and ligases
3. Vectors and Markers
4. Reverse transcriptase

Q. There is a restriction endonuclease called EcoRI. What does "co" part in it stand for?

1. coli
2. colon
3. coelom
4. coenzyme

Q. Eco RI is

1. a restriction enzyme
2. a plasmid
3. used to join two DNA fragments
4. the abbreviation for bacterium Escherichia coli

Q.

The restriction enzyme is:

1. Are required in vectorless direct gene transfer

2. Endonuclease which cleaves DNA at specific sites

3. Make DNA complementary to an existing DNA or RNA

4. Cut or join DNA fragments

Q. Eco RI is

1. terminal transferase
2. restriction enzyme
3. vector virus
4. vector plasmid

Q. Select the correct increasing order of organisation

1. Chromosome - DNA - Chromatin - Nucleosome
2. DNA - Chromosome - Chromatin - Nucleosome
3. Chromatin - Nucleosome - Chromosome - DNA
4. DNA - Nucleosome - Chromatin - Chromosome