Genetic Engineering

Q.

Asexual reproduction results in

1. Identical copies of the parent
2. Extreme differences between parent and offspring
3. Characteristics of offspring similar to two different parents
4. A new species

Q.

The first step in genetic engineering is:

1. Isolation of RNA

2. Isolation of protein
   

3. Purification of protein

4. Isolation of genetic material

Q. What are the basic principles of genetic engineering ?

Q. From the below list, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
(i) Transformbacteria with recombinant DNA molecule.
(ii) Cut the plasmid DNA using restriction enzymes.
(iii) Extract plasmid DNA from bacterial cells.
(iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to nonplasmid DNA.

1. (iv), (v), (i), (ii), (iii)
2. (iii), (ii), (iv), (v), (i)
3. (iii), (iv), (v), (i), (ii)
4. (ii), (iii), (v), (iv), (i)

Q. Restriction enzymes can cleave the DNA at any site randomly.

1. True
2. False

Q.

Which of the following is the correct match of enzymes with their rule in genetic engineering?

1. DNA polymerase - cutting the DNA

2. DNA ligase - helps in DNA replication

3. Restriction enzyme - helps in DNA replication.

4. Restriction enzyme - cutting the DNA

Q. Which of the following is not a restriction endonuclease?

1. Eco RI
2. Pst I
3. DNAse I
4. Hind III

Q. A kind of biotechnology involving manipulation of DNA is

1. DNA replication
2. Genetic Engineering
3. Denaturation
4. Renaturation

Q. Genetic engineering was developed during

1. 1970
2. 1980
3. 1990
4. 2000

Q. Which of the following statement is not true for a clone?

1. Clones are descended from a single parent
2. Identical twins are not clones
3. Clone is a result of sexual reproduction
4. Both (a) and (c)

Q.

Genetic engineering is possible because:

1. We can see DNA by an electron microscope.

2. We can cut DNA at specific sites by endonuclease like DNAase I.
   

3. Restriction endonuclease purified from bacteria can be used in vitro.
   

4. The phenomenon of transduction in bacteria is well understood.

Q.

Which of the following is the correct match of enzymes with their rule in genetic engineering?

1. DNA polymerase - cutting the DNA

2. 
   Restriction enzyme - cutting the DNA

3. 
   DNA ligase - helps in DNA replication

4. 
   Restriction enzyme - helps in DNA replication.
   

Q.

The technique in which a foreign DNA is coated on the surface of tungsten or gold particles and shot into the target cells is known as:

1. Microinjection

2. Chemical – mediated gene transfer
   

3. Particle gun

4. Electroporation

Q.

Advancement in genetic engineering has been possible due to

1. Oncogenes

2. Exonucleases

3. Transposons

4. Endonucleases

Q. Antibiotic resistance is passed from one bacterium to the other by the process of ______.

1. Chemotherapy
2. Cloning
3. Restriction digestion
4. Conjugation

Q. Transfer of any gene into a completely different organism can be done through _____.

1. tissue culture
2. DNA replication
3. genetic engineering
4. conjugation

Q. Regarding assertion and reason choose the correct option.
Assertion [A] : Insulin can be produced in bacterial cells.
Reason [R] : Genetic engineering techniques can be used to transfer genes across species.

1. [A] is true and [R] is false
2. Both [A] and [R] are true and [R] is a correct explanation to [A]
3. [A] is false and [R] is true
4. Both [A] and [R] are true but [R] is not a correct explanation to [A]

Q. explain semi- conservative replication of eukaryotic DNA

Q. The first step in genetic engineering is

1. Isolation of protein
2. Purification of protein
3. Isolation of genetic material
4. Isolation of RNA