Recombinant DNA Technology

Q.

What are the methods of genetic recombination in prokaryotes?

Q. The vectorless gene transfer include

1. Gene gun
2. Microinjection
3. Electroporation
4. All the above

Q. Match the following
$\begin{array}{cc}ColumnI& ColumnII\\ \text{P. Gene cloning}& \text{i. DNA from multiple sources.}\\ \text{Q. Recombinant DNA}& \text{ii. Copies of gene of interest.}\\ \text{R. Genetic engineering}& \text{iii. Vegetative propagation.}\\ \text{S. Clones}& \text{iv. Techniques used to modify the genome of an organism.}\end{array}$

1. 
2. 
3. 
4. 

Q. In a genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Which of the following can be used as a DNA vectors?

1. Bacteriophage
2. Plasmid
3. Plasmodium
4. Both A and B

Q.

What are the three steps of comprehensive GMO analysis techniques?

Q. Who were the pioneers of recombinant DNA (rDNA) technology?

1. Walter Sutton and Avery
2. Har Gobind Khorana
3. James D Watson
4. Stanley Cohen and Herbert Boyer

Q.

Name one technique used to modify a genome.

Q. Two bacteria found to be very useful in genetic engineering are ______________

1. Nitrosomonas & Klebsiella
2. Nitrobacter & Azotobacter
3. Rhizobium & Diplococcus
4. Escherichia & Agrobacterium

Q. Regarding assertion and reason, select the correct option

Assertion: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote)
Reason: Both bacteria and yeast multiply very fast to form huge population which express the desired gene.

1. Assertion is true but the Reason is false
2. Both Assertion and Reason are true and Reason is correct explanation of the Assertion.
3. Both Assertion and Reason are true but Reason is not the correct explanation of Assertion
4. Both Assertion and Reason is false

Q. Which was the first product contributed to human kind by science of biotechnology in early 1972?

1. Somatotropin
2. Insulin
3. Cytokines
4. Erythropoietin

Q. What are plus strand and negative strand DNA and RNA?

Q. Which one of the following techniques made it possible to genetically engineer living organisms ?

1. Recombinant DNA technique
2. Hybridization
3. X-Ray diffraction
4. Heavier isotope labeling

Q. The technique used in biotechnology is

1. hybridization
2. Both a & b
3. Recombinant DNA technology
4. None of these