Restriction Enzyme

Q. Bacteria protect themselves from viral attack by producing ________

1. Endonuclease
2. Exonuclease
3. DNA ligase
4. Gyrase

Q.

What is the role of PCR in the amplification of DNA strands?

Q. The specific DNA sequence where Eco – RI cuts is

1. GATTCG
2. GAATTC
3. GTTCAA
4. TTCCAA

Q. Which of the following is a restriction endonuclease ?

1. Hind II
2. Protease
3. DNase I
4. RNase

Q. Which of the following is specifically used in genetic engineering?

1. Ligase
2. Gyrase
3. Restriction endonuclease
4. DNA polymerase

Q. Which one is regarded as a molecular scissor in biotechnology?

1. Reverse transcriptase
2. Restriction endonuclease
3. Taq polymerase
4. Topoisomerase

Q. GAATTC is the reorganization site for which of the following restriction endonuclease?

1. Bam I
2. Hae III
3. Hin d III
4. Eco R I

Q. Restriction endonucleases are enzymes

1. make cuts at specific positions within the DNA molecule
2. recognise a specific nucleotide sequence for binding of DNA ligase
3. restrict the action of the enzyme DNA polymerase
4. remove nucleotides from the ends of the DNA molecule

Q. More advancement in Genetic engineering during the 1970s happened due to _________

1. Zymase
2. Restriction endonuclease
3. Protease
4. Reverse transcriptase

Q. Endonuclease is used in

1. Genetic engineering
2. Transcription
3. Translation
4. None of these

Q. Genetic engineering is possible because

1. we can cut DNA at specific sites by endonucleases like DNase I
2. restriction endonucleases purified from bacteria can be used in vitro
3. the phenomenon of transduction in bacteria is well understood
4. we can see DNA by electron microscope

Q. Restriction endonucleases are enzymes which

1. Make cuts at specific positions within the DNA molecule
2. Recognize a specific nucleotide sequence for binding of DNA Ligase
3. Restrict the action of enzyme DNA polymerase
4. Remove nucleotides from the ends of DNA molecule

Q. A graduate student working on a project is attempting to isolate a gene from yeast. He identifies the restriction sites to cut the gene of interest out. But after several attempts, fails to do so. What could be the possible reason for the failure?

1. Restriction enzyme used has recognition sites in the gene
2. The gene of interest in yeast is methylated
3. Yeast genome is too complex containing overlapping genes
4. The gene of interest is too big to isolate

Q. Which of the following is not a tool in genetic engineering?

1. Restriction endonucleases and ligases
2. Exonuclease
3. Reverse transcriptase
4. Vectors and Markers

Q. In Cohen and Boyer's recombinant DNA experiments, restriction endonucleases were used to

1. Isolate fragments of cloned bacterial plasmids.
2. Isolate fragments of frog DNA that contained an rRNA gene.
3. All the above
4. Cleave the bacterial plasmid.

Q. In the resistriction endonuclease EcoRI, what does ‘co’ stand for?

1. Coelom
2. Coenzyme
3. Coli
4. Colon

Q. In the resistriction endonuclease EcoRI, what does ‘co’ stand for?

1. Coenzyme
2. Coelom
3. Coli
4. Colon

Q. What process does the following diagram represent?

1. Lysogenic cycle
2. Action of endonucleases
3. Lytic cycle
4. Cloning

Q. Restriction endonucleases are

1. used for in vitro DNA synthesis
2. used in genetic engineering
3. synthesized by bacteria
4. present in mammalian cells for degradation of DNA.