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Question

Restriction endonucleases are enzymes which

A
Make cuts at specific positions within the DNA molecule
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B
Recognize a specific nucleotide sequence for binding of DNA Ligase
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C
Restrict the action of enzyme DNA polymerase
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D
Remove nucleotides from the ends of DNA molecule
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Solution

The correct option is A Make cuts at specific positions within the DNA molecule

Restriction endonucleases recognises a specific DNA base sequence (recognition sequence, recognizion site, restriction sequence or restriction site having palindromic sequence) and cleaves both the strands of DNA at or near the site. The enzyme cuts the DNA, generating restriction fragments with overhanging ends (sticky ends) or blunt ends.


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