Screening of Recombinant Cell

Q. The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of:

1. Non-recombinant bacteria containing beta-galactosidase
2. Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
3. Insertional inactivation of alpha-galactosidase in recombinant bacteria
4. Inactivation of glycosidase enzyme in recombinant bacteria

Q.

Which of the following is true about insertional inactivation of the β-galactosidase coding sequence?

1. The non-transformants exhibit blue coloured

2. The transformants do not exhibit any colour

3. The Non-transformants do not exhibit any colour

4. The transformants exhibit blue coloured

Q. If a recombinant DNA bearing gene for ampicillin resistance is transferred into E. coli cells and the host cells are spread on agar plates containing ampicillin, then

1. both transformed and untransformed recipient cells will die
2. both transformed and untransformed recipient cells will grow
3. transformed recipient cells will grow and untransformed recipient cells will die
4. transformed recipient cells will grow and untransformed recipient cells will grow