Genetic Engineering Requirements

Q.

What is the wobble effect?

Q.

Define genetic engineering.

Q.

What is DNA restriction and modification?

Q.

Which of the following is an undifferentiated cell?

1. Foetal cells

2. Zygote

3. Blood cells

4. Neurons

Q. One of the key factors that makes the plasmid a vector in genetic engineering is that

1. it is resistant to restriction enzymes
2. it is resistant to antibiotics
3. it is able to carry a foreign gene
4. its ability to cause infection in the host.

Q.

Biotechnology includes which among the following main area

1. Use of biomolecules like DNA and protein in human welfare

2. Use of productivity of cells. Eg. production of antibiotics and vaccines

3. USe of genetics and non-genetic technique.

4. All of the above

Q.

What is replication of DNA.

Q.

What is a type 2 restriction enzyme?

Q.

What are hybrid seeds? List their useful and harmful effects.

Q.

Name one demonstration wherein stability as a property of genetic material was evident.

Q.

Production of a replica of any cell or organ or entire organism is called

1. None of the above

2. Gene Therapy

3. White biotechnology

4. Cloning

Q.

What is the requirement for screening DNA libraries by hybridization?

Q.

What is a type 1 restriction enzyme?

Q.

Cloning is an experimental technique, where in a group of morphologically and genetically identical organisms are produced.

1. True

2. False

Q. What is speciation and what are factors regarding genetic drift, etc Explain each and every factor .
.

Q.

Is plasmid a cloning vector?

Q.

Genetic engineering is the technique in which the genetic constitution of an organism is altered by introducing new genes into its chromosomes.

1. True

2. False

Q.

In which of the following was genetic engineering first employed commercially?

1. To develop human DNA using bacteria.

2. To develop genetically modified plants with high resistance.

3. To develop human insulin using bacteria.

4. To develop genetically modified plants with high productivity.

Q.

Which Vector Can Clone A Small Fragment Of DNA?

Q. Transfer of any gene into a completely different organism can be done through _____.

1. tissue culture
2. conjugation
3. DNA replication
4. genetic engineering

Q. The enzymes which are commonly used in genetic engineering are

1. Endonuclease and ligase
2. Restriction endonuclease and polymerase
3. Ligase and polymerase
4. Restriction endonuclease and ligase

Q. Write an essay on recombinant DNA technology.

Q. Two bacteria found to be very useful in genetic engineering experiments are

1. Nitrosomonas and Klebsiella
2. Escherichia and Agrobacterium
3. Nitrosomonas and Azotobacter
4. Rhizobium and Diplococcus

Q. The transfer of protein from electrophoric gel to nitrocellulose membrane is known as

1. Transferase
2. Northern blotting
3. Western blotting
4. Southern blotting

Q. Repressor protein is produced by

1. Regulator gene
2. Structural gene
3. Promoter gene
4. Operator gene

Q. Who among the following, discovered the enzyme restriction endonuclease?

1. Hamilton Othanel Smith
2. F. Jacob
3. Andre Lwoff
4. Sir Godfrey Hounsfield

Q. Ti plasmid used in genetic engineering is obtained from _________________.

1. Bacillus thuringiensis
2. Agrobacterium rhizogenes
3. Agrobacterium tumefaciens
4. Pseudomonas syringae

Q. Research projects:

1. cloning
2. identical/ non identical twins
3. triplets/ quadruplets
4. surrogacy
5. IVF

Q. Manipulation of DNA to change its structure is called genetic engineering.

1. True
2. False

Q. Transfer of any gene into a completely different organism can be done by

1. Genetic engineering
2. Cloning
3. Coning a gene
4. Tissue culture