Electroporation

Q. Biolistic (gene) gun is suitable for

1. disarming pathogen vectors
2. transformation of plant cell
3. constructing recombinant DNA by joining with vectors
4. DNA fingerprinting

Q.

What is the smallest virus or bacteria?

Q. Define transformation in Griffith's experiment. Discuss how it helps in the identification of DNA as the genetic material.

Q. Polyploidy is caused due to

1. failure of cytokinesis
2. nondisjunction of chromatids
3. chromatin condensation
4. misalignment at the metaphase plate

Q. DNA fragments separated by gel electrophoresis are shown. Mark the correct statement-

1. Band 3 contains more positively charged DNA molecule than 1
2. Band 3 indicates more charge density than 1 and 2
3. Band 1 has longer DNA fragment than 2 and 3
4. All the bands have equal length and charges but differ in base composition

Q. Match column I with column II, and choose the correct combination from the options given below.

	Column I		Column II
a.	DNA Ligase	1.	Agrobacterium
b.	Ti-plasmid	2.	Joining the DNA fragments
c.	Taq polymerase	3.	$\beta$-galactosidase
d.	Insertional inactivation	4.	Thermostable DNA polymerase

1. $a-2,b-1,c-3,d-4$
2. $a-1,b-2,c-3,d-4$
3. $a-2,b-1,c-4,d-3$
4. $a-3,b-4,c-2,d-1$

Q. The organism which is used for gene transfer in higher organism is ___________.

1. Agrobacterium tumefaciens
2. E. coli
3. Acetobacter
4. Bacillus thuringiensis

Q.

What does the Artificial Chromosome hold?

Q. Due to difficulty in inactivation of antibiotics, alternate selectable markers developed to differentiate recombinants from non-recombinants on the basis of ability to

1. All are correct
2. Produce blue colour colonies due to insertional
   inactivation
   
3. Produce colour in the presence of a chromogenic
   substrate
   
4. Produce bright orange colour in the presence of a
   chromogenic substrate
   

Q. Cohen and Boyer isolated an antibiotic resistance gene by cutting out a piece of DNA from a plasmid having antibiotic resistance in the year

1. 1972
2. 1963
3. 1967
4. 1982

Q. While creating the first Recombinant DNA why was it necessary for linking the gene of interest with the plasmid of Salmonella typhimurium when E coli already have their own plasmids??? Why can't we directly introduce our gene of interest in ecoli medium so that our gene of interest is directly in the plasmid of ecoli itself??

Q. The organism which is used for gene transfer in higher organism is ___________.

1. Agrobacterium tumefaciens
2. E. coli
3. Acetobacter
4. Bacillus thuringiensis

Q. Polyethylene glycol method is used for

1. biodiesel production
2. seedless fruit production
3. energy production from sewage
4. gene transfer without a vector

Q. Agrobacterium tumifaciens is pathogenic to _____________.

1. monocots
   
2. dicots
3. gymnosperms
4. bryophytes

Q. The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as:

1. Conjugation
2. Translation
3. Transformation
4. Transduction

Q. The probe used initially by Alec Jeffreys during development of DNA fingerprinting was

Q. The organism, which is used for gene transfer in higher organism is

1. Agrobacterium tumefaciens
2. E.coli
3. Acetobacter aceti
4. Bacillus thuringiensis

Q. The genes for antibiotic resistance are found in

1. Plasmid
2. Nucleus
3. Cell membrane
4. Chromosome

Q. Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells):

1. Both sense and anti-sense RNA
2. A particular hormone
3. An antifeedant
4. A toxic protein

Q. Would you explain how a baby can born with two mothers and one father(regarding mitochondria)?
how we can inherit only mother's mitochondrial DNA..Why not father's???

Q.

Why E.coli is used much more time as a host cell and why not any other bacteria?

Q. Which of the following is not a method of gene transfer in Bacteria?

1. Transformation
2. Transduction
3. Conjugation
4. Gametangia contact

Q. DNA is unique in that it has

1. Nitrogen bases
2. Ability to replicate
3. Ability to withstand heat
4. Ability for replication and transcription

Q. Transfer of DNA bands from agarose gel to nitrocellulose or nylon membrane is

1. Western transfer
2. Southern transfer
3. Northern transfer
4. Eastern transfer
5. Gene transfer

Q. Polyethylene glycol method is used for :

1. Energy production from sewage
2. Biodiesel production
3. Gene transfer without a vector
4. Seedless fruit production

Q. Explain any two methods for introduction of recombinant DNA into host cell.

Q. Transfer of genetic material into a bacterial cell through a viral vector is known as

1. Transformation
2. Transduction
3. Transfection
4. Translation

Q. Which of the following methods can be used to make the plasmid vector enter the competent cell?

1. Electroporation
2. Potassium chloride treatment
3. Heat shock
4. Ultra diluted HCl

Q. Most suitable method of introducing alien DNA into a plant cell is

1. Biolistics
2. Micro-injection
3. Lipofection
4. Heat shock method

Q. Direct gene transfer was first used in

1. Potato plant
2. Tobacco plant
3. Tomato plant
4. None of the above