Functions of RNA

Q. Read the following statements regarding transcription.
1. RNA polymerase initiates the process of transcription with the help of sigma factor.
2. RNA polymerase also facilitates opening of the DNA double helix in prokaryotes.
3. Transcription requires primer
4. In bacteria, there are three types of RNA polymerases.
How many of the above statements are incorrect?

1. 2
2. 3
3. 1
4. 4

Q. Which of the following statements are incorrect?
i. RNA acts as a genetic material as well as a catalyst
ii. RNA is more stable than DNA
iii. RNA has evolved from DNA
iv. The process of translation has evolved around RNA

1. i and ii
2. ii and iii
3. iii and iv
4. iv and i

Q. RNA polymerase I: _________ :: _________ : Transcribes tRNA
[1 mark]

1. Transcribes sRNA ; RNA polymerase II
2. Transcribes hnRNA ; RNA polymerase III
3. Transcribes snRNA ; RNA polymerase II
4. Transcribes rRNA ; RNA polymerase III

Q.

RNA polymerase II is responsible for transcription of

1. snRNA

2. tRNA

3. rRNA

4. hnRNA

Q. Types of RNA polymerase required in nucleus for RNA synthesis are:

1. 4
2. 1
3. 2
4. 3

Q.

Consider the below situation and select the correct option.

During an experiment conducted by a group of scientists in a eukaryotic cell they deliberately removed the RNA polymerase III from nucleoplasm. This act will most severely affect the synthesis of

1. t RNA
2. hn RNA
3. m RNA
4. r RNA

Q. Eukaryotic RNA polymerase-III catalyses the synthesis of

1. m-RNA
2. 28S rRNA
3. 18S rRNA
4. 5S rRNA

Q. The cofactor for the enzyme carboxypeptidase is

1. copper
2. iron
3. zinc
4. manganese

Q. Due to discovery of which of the following in 1980, the evolution was termed as RNA world:

1. m-RNA, t-RNA-r-RNA synthesise proteins
2. In some virus RNA is genetic material
3. RNA has enzymatic property
4. RNA is not found in all cells

Q. The enzyme involved in eukaryotic transcription:

1. RNA polymerase-I
2. RNA polymerase-II
3. RNA polymerase-III
4. All of the above

Q.

How did DNA evolve?

Q. Which of the following statements is NOT true for eukaryotic transcription?

1. Core promoter determines the location of start point
2. Transcription factors recognize the promoter
3. Transcription factors are cis-regulatory proteins
4. Transcription termination is different for different RNA polymerases

Q. Which of the following types of RNA will not be formed in the absence of RNA polymerase II?

1. rRNA
2. snRNA
3. tRNA
4. hnRNA

Q.

Assuming that the method of protein synthesis remained same what would be the number of nucleotide sequences in a codon if evolution was so progressed that instead of 20 amino acid there were 96 and instead of 4 nitrogen bases there were 12

Q. Consider the following statements regarding eukaryotes.
I. RNA polymerase I transcribes rRNAs
II. RNA polymerase II transcribes snRNAs
III. RNA polymerase III transcribes hnRNA
IV. RNA polymerase III transcribes tRNA
Which of the statements given above are correct?

1. I and II
2. I and III
3. I and IV
4. I, II, III

Q.

Is RNA self splicing?

Q.

Why is DNA polymerase 3 important?

Q.

What is the role of RNA Polymerase in elongation?

Q. (a) Describe the process of transcription in bacteria.

(b) Explain the processing the hnRNA which needs to undergo before becoming functional mRNA eukaryotes. [5]

Q. Which of the following is evidence against the statement “All enzymes are proteins”?

1. Ribozyme
2. Arylsulfatase
3. Nitroreductase
4. Dehydrogenase

Q.

Why do we need DNA polymerase?

Q. explain the role of DNA dependent RNA polymerase in initiation, elongation and termination during transcription in bacterial cell

Q. Name the enzyme that catalyses the transcription of $hnRNA$.

Q. The primary transcript is much longer than mature mRNA because of the presence of introns in the former.

1. True
2. False

Q. Eukaryotic RNA polymerase-III catalyses the synthesis of

1. m-RNA
2. 28S rRNA
3. 18S rRNA
4. 5S rRNA

Q. Sequence of amino acid is represented by which of the following structures of protein?

1. Secondary structure
2. Primary structure
3. Tertiary structure
4. Quaternary structure

Q. What particular aspect of DNA replication causes the leading strand to be constructed more quickly than the lagging strand?

1. DNA replication requires primase to assemble a primer.
2. To begin replication, the origin of replication must first be opened by initiator proteins.
3. During replication, DNA can only be built in a 5' to 3' direction.
4. DNA replication is a semiconservative process that depends on complementary base pairing.

Q. The differences between mRNA and tRNA are that
(i) mRNA has more elaborated 3-dimens~onaI structure due to extensive base-pairing
(ii) tRNA has more elaborated 3-dimensional Structure due to extensive base-pairing
(iii) tRNA is usually smaller than mRNA
(iv) mRNA bears anticodon but iRNA has codons.

1. (i) and (ii)
2. (ii) and (iii)
3. (i), (ii) and, (iii)
4. (i), (ii), (iii) and (iv)

Q.

Transcription is a process by which

1. Amino acids are joined to form polypeptides.
2. An RNA molecule is synthesized from a DNA template.
3. An RNA molecule is synthesized within a ribosome.
4. Two daughter strands of DNA are synthesized.

Q. A non-proteinaceous enzyme is

1. lysozyme
2. deoxyribonuclease
3. Ribozyme
4. ligase