HGP

Q. In an experiment, E.coli with ${}^{15}$N DNA were taken. They were transferred to ${}^{14}$N medium to grow. What percentage of DNA will have ${}^{15}$N${}^{15}$N, ${}^{15}$N${}^{14}$N and ${}^{14}$N${}^{14}$N after 60 minutes?

1. 0%, 50%, 50%
2. 50%, 0%, 50%
3. 0%, 25%, 75%
4. 75%, 25%, 0%

Q.

What is chromosome mapping?

Q.

How much DNA is present in a single human cell?

Q. Which of the following is not a goal of HGP?

1. Storage of information in databases.
2. Physical mapping of the human genome.
3. Addressing ELSI.
4. Identification of non-protein coding genes.

Q.

Why is the human genome project called a mega project?

Q.

What is average heterozygosity?

Q. The total number of genes in humans is far less $(<25,000)$ than the previous estimate (upto 1, 40, 000 genes). Comment.

Q.

Genes when close together on a chromosome are known as:

1. Linkage

2. Mutation

3. Translation

4. Transcription

Q. Identify the correct sequence of the various steps involved in the process of sequencing the human genome.

1. Fragmentation of DNA
2. Cloning of DNA fragments using vectors
3. Sequencing
4. Isolation of DNA

1. 1, 4, 3, 2
2. 4, 1, 3, 2
3. 4, 1, 2, 3
4. 4, 2, 3, 1

Q. Refer the given sequence of steps and select the correct option.

$DNA\stackrel{\left(i\right)}{\to }hnRNA\stackrel{\left(ii\right)}{\to }mRNA\stackrel{\left(iii\right)}{\to }Proteins$

1. $\begin{array}{ccc}\left(i\right)& \left(ii\right)& \left(iii\right)\\ \text{Replication}& \text{Transcription}& \text{Translation}\end{array}$
2. $\begin{array}{ccc}\left(i\right)& \left(ii\right)& \left(iii\right)\\ \text{Replication}& \text{Processing}& \text{Translation}\end{array}$
3. $\begin{array}{ccc}\left(i\right)& \left(ii\right)& \left(iii\right)\\ \text{Transcription}& \text{Splicing}& \text{Translation}\end{array}$
4. $\begin{array}{ccc}\left(i\right)& \left(ii\right)& \left(iii\right)\\ \text{Transcription}& \text{Replication}& \text{Translation}\end{array}$

Q.

Which of the following is the correct sequence of the units of genetics arranged in descending order of size?

1. Gene →Cistron →Muton →Recon

2. Gene → Cistron → Recon → Muton

3. Gene →Muton → Cistron → Recon

4. Gene →Recon → Cistron →Muton

Q.

Do genes evolve?

Q. (a) List the two methodologies which were involved in human genome project. Mention how they were used.

(b) Expand "YAC' and mention what was it used for. [3]

Q.

What percentage of the human genome is coding?

Q.

What makes up the human genome?

Q. Who discovered that the DNA could be sequenced and the correct series of nucleotides on a fragment of DNA could be identified?

1. Frederick Sanger
2. James Watson
3. Ray Wu
4. Rosalind Franklin

Q. In a maternity clinic, the authorities are not able to hand over the two newborns to their respective parents. Name and describe the technique to sort out this problem. [3]

Q.

Which of the following is not a part of modern genetic engineering?

1. DNA profiling

2. Gene mapping

3. DNA finger printing
4. X-ray diffraction

Q. What do '$Y$' and '$B$' stand for in '$YAC$' and '$BAC$' used in Human Genome Project (HGP)? Mention their role in the project.

Q. What is Human genome project ? Briefly write about the salient features of Human Genome project.

Q. The main goal of human genome project was

1. find the total number of chromosomes in the nucleus
2. sequence all the DNA base pairs in the genome
3. find number of nitrogenous base pairs in the genome
4. find the number of genes of one chromosome in the genome

Q. State any three goals of the human genome project.

Q. What does abbreviation HGP stand for ?

Q. The first organism to have its genome sequenced is __.

Q. The first organism to have its genome sequenced is __.

Q. Humen chromosome with highest and feast genes

Q. Which among the following is a prospect/implication of the Human Genome Project?

1. It helps to determine cancer causing genes
2. It holds the prospects of a vast database about our genetic identity
3. It tends to bring about technological advances in the field of biology for the benefit of mankind
4. All of these

Q. The human genome contains (a) billion nucleotide bases. The average gene consists of (b) bases. Which option is correct for a & b?

1. $a=5,b=3000$
2. $a=3,b=3000$
3. $a=3,b=2000$
4. $a=4,b=3000$

Q. If the arrangement of genes A, B, C and D on any of the chromosomes is ‘ABDC’, then which among the following is the incorrect statement?

1. The approximate distance between genes A&B can be 20m.u, B&C 30m.u, C&D 10m.u and A&C 50m.u.
2. The approximate distance between genes A&B can be 15m.u, B&C 35m.u, C&D 5m.u and A&C 50m.u.
3. The approximate distance between genes A&B can be 31m.u, B&C 10m.u, C&D 5m.u and A&C 4m.u.
4. The approximate distance between genes A&B can be 22m.u, B&C 28m.u, C&D 8m.u and A&C 50m.u.

Q.

Which of these animals are more closely related to each other than the others ?

Animal A - 35 STRs

Animal B - 70 STRs

Animal C - 45 STRs

Animal D - 90 STRs

1. Animals A and B
2. Animals B and D
3. Animals A and C
4. Animals B and D