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Question

Identify the correct sequence of the various steps involved in the process of sequencing the human genome.


A
1, 4, 3, 2
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B
4, 1, 3, 2
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C
4, 1, 2, 3
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D
4, 2, 3, 1
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Solution

The correct option is C 4, 1, 2, 3
The process of sequencing the human genome occurs in the following steps:
  • Isolation: Isolation of the entire DNA from the cell to make it free from other biomolecules like proteins, carbohydrates, lipids.
  • Fragmentation: DNA is a very long polymer, and thus it cannot be completely sequenced at once. Thus, it is cut into smaller fragments.
  • Cloning: Once fragmentation is done, the DNA fragments are cloned in the suitable host using specialised vectors.
  • Sequencing: Once cloned, the DNA fragment is amplified so that it can be sequenced easily.
Hence, the correct sequence of the various steps involved in the process of sequencing the human genome are
4, 1, 2, 3.

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