Question

Recombinant DNA technology involves several steps in specific sequence. Find out the correct sequence.

a. Fragmentation of DNA
b. Culturing the host cells in a medium at large scale
c. Ligation of DNA fragment into a vector
d. Extraction of the desired product
e. Isolation of DNA
f. Isolation of desired DNA fragment
g. Transferring the recombinant DNA into the host

• A. e–a–f–g–c–b–d
• B. e–f–a–c–g–b–d
• C. a–e–c–f–g–d–b
• D. e–a–f–c–g–b–d

Answer 1

The correct option is D e–a–f–c–g–b–d

Steps of recombinant DNA technology:

1. Isolation of genetic material i.e., isolation of the DNA fragments or genes to be cloned
2. Restriction enzyme digestion
3. Amplification
4. Ligation
5. Insertion of recombinant DNA into host i.e., insertion of the isolated gene in a suitable plasmid vector
6. Transformation i.e., introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)
7. Obtaining foreign gene product i.e., multiplication/expression of the introduced gene in the host
8. Down streaming process i.e., extraction of the desired product

So the correct answer is option "d".