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Question

Identify the correct sequence/steps involved in recombinant DNA technology:
A) Culturing the host cells in a medium at large scale
B) Isolation of DNA
C) Isolation of a desired DNA fragment
D) Transferring the recombinant DNA into the host
E) Ligation of the DNA fragment into a vector
F) Fragmentation of DNA by restriction endonucleases
G) Extraction of the desired product

A
B F C E D A G
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B
B C F D E A G
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C
B E C F A D G
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D
B F D A C E G
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Solution

The correct option is A B F C E D A G
The following are the steps involved in processing of recombinant DNA technology:
i) Isolation of DNA
ii) Fragmentation of DNA by restriction endonucleases
iii) Isolation of a desired DNA fragment
iv) Ligation of the DNA fragment into a vector
v) Transferring the recombinant DNA into the host
vi) Culturing the host cells in a medium at large scale
vii) Extraction of the desired product

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