Question

Identify the correct sequence/steps involved in recombinant DNA technology:
A) Culturing the host cells in a medium at large scale
B) Isolation of DNA
C) Isolation of a desired DNA fragment
D) Transferring the recombinant DNA into the host
E) Ligation of the DNA fragment into a vector
F) Fragmentation of DNA by restriction endonucleases
G) Extraction of the desired product

• A. B $\to$ F $\to$ C $\to$ E $\to$ D $\to$ A $\to$ G
• B. B $\to$ C$\to$ F $\to$ D$\to$ E $\to$ A $\to$ G
• C. B $\to$ E$\to$ C $\to$ F $\to$ A $\to$ D $\to$ G
• D. B $\to$ F $\to$ D $\to$ A $\to$ C $\to$ E $\to$ G

Answer 1

The correct option is A B $\to$ F $\to$ C $\to$ E $\to$ D $\to$ A $\to$ G

The following are the steps involved in processing of recombinant DNA technology:
i) Isolation of DNA
ii) Fragmentation of DNA by restriction endonucleases
iii) Isolation of a desired DNA fragment
iv) Ligation of the DNA fragment into a vector
v) Transferring the recombinant DNA into the host
vi) Culturing the host cells in a medium at large scale
vii) Extraction of the desired product