Question

Some of the steps of DNA fingerprinting are given below. Identify the correct sequence from the options given.

I. Electrophoresis of DNA fragments.

II. Hybridisation with DNA probe.

III. Digestion of DNA of RENs.

IV. Autoradiography.

V. Blotting of DNA fragments to nitrocelluose membrane.

• A. III, I, V, II, IV
• B. I, III, V, IV, II
• C. III, I, II, V, IV
• D. I, V, III, II, IV

Answer 1

The correct option is A

III, I, V, II, IV

DNA fingerprinting is a technique involved the identification of differences in repetitive DNA. It is used as a tool in the forensic investigation for solving paternity disputes, etc.

Correct steps or sequence of DNA fingerprinting are:

Digestion of DNA by restriction endonuclesase $\to$ Separation or electrophoresis of DNA fragments $\to$ Blotting of DNA fragments to nitrocellulose membrane $\to$ Hybridisation with DNA probes $\to$ Detection of hybridised DNA fragments by autoradiography.