Question

Some of the steps of DNA fingerprinting are given below. Identify their correct sequence from the options given:
A. Electrophoresis of DNA fragments
B. Hybridization with DNA probe
C. Digestion of DNA by restriction endonucleases
D. Autoradiography
E. Blotting of DNA fragments nitrocellulose membrane

• A. C – A – B – E – D
• B. C – A – E – B – D
• C. A – E – C – B – D
• D. A – C – E – D – B

Answer 1

The correct option is B C – A – E – B – D

The following are the steps of DNA fingerprinting:
(i) Isolation of DNA from the organism
(ii) Digestion of DNA by restriction endonucleases
(iii) Separation of digested DNA fragments by electrophoresis
(iv) Transferring (blotting) of separated DNA fragments to synthetic membranes like nitrocellulose or nylon membranes
(v) Hybridisation of DNA by using probe
(vi) Detection of hybridised DNA fragments by autoradiography