Hydrolases

Q. Why Hind II is the first restriction enzyme not Hind I?

Q.

Which enzyme in transamination catalyses reactions?

Q. Which class of enzyme converts glucose – 6 – phosphate to fructose – 6 – phosphate?

1. dehydrogenases
2. Tranferases
3. Hydrolases
4. Isomerases

Q.

Which of the following reactions is not enzyme mediated in biological system ?

(a) Dissolving $C{O}_2$ in water (b) Unwinding the two strands of DNA

(c) Hydrolysis of sucrose (d) Formation of peptide bond

Q. Which of the following statements are true about lac operon?
I. In the absence of lactose, the repressor binds with the operator region.
II. All three structural genes in the operon are not essential for the metabolism of lactose.
III. The operator is a negative regulatory site bound by the lac repressor protein.

1. II and III
2. I and II
3. II only
4. I and III

Q.

How are proteins used to make ATP?

Q. Enzymes catalysing the breakdown of larger molecules into smaller molecules are

1. hydrolases
2. isomerases
3. ligases
4. both (a) and (c)

Q.

What class of enzyme is succinate dehydrogenase?

Q. Which one of the following hydrolyzes internal phosphodiester bonds in a polynucleotide chain:

1. Lipase
2. Exonuclease
3. Endonuclease
4. Protease

Q. Most of the digestive enzymes belong to the class of ________.

1. Transferases.
2. Hydrolases
3. Lyases
4. Oxidoreductases

Q. (a)What do you understand by the term apoenzyme?
(b)Distinguish between isoenzyme and coenzyme.
(c)Distinguish between apoenzyme and coenzyme.
(d)Differentiate between enzyme and isoenzyme.

Q. Which of the following statements are true about lac operon?
I. In the absence of lactose, the repressor binds with the operator region.
II. All three structural genes in the operon are not essential for the metabolism of lactose.
III. The operator is a negative regulatory site bound by the lac repressor protein.

1. II and III
2. I and II
3. II only
4. I and III

Q. Assertion :Each enzyme has a substrate binding site in its molecule which forms highly reactive enzyme- substrate complex. Reason: The enzyme-substrate complex is long- lived and dissociates into its product and unchanged enzyme.

1. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
2. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
3. Assertion is correct but Reason is incorrect
4. Both Assertion and Reason are incorrect

Q. Most of the digestive enzymes belong to the class of ________.

1. Lyases
2. Hydrolases
3. Oxidoreductases
4. Transferases.

Q. Template/lock and key theory of enzyme action is supported by the fact that

1. Enzymes speed up reaction
2. Enzymes determine the direction of reaction
3. Compounds similar to substrate inhibit enzyme activity
4. Enzymes occur in living beings and speed up certain reactions

Q. Which one is a non reducing commercial sugar?

1. Sucrose
2. Fructose
3. Lactose
4. Glucose

Q. The amine acid acceptor arm of the tRNA is at its?

1. DHU $100p$
2. J $\psi$ C $100p$
3. $5^{\prime }$ end
4. None

Q. Which of the following is an allosteric enzyme?

1. Hexokinase
2. Phosphofructokinase
3. Succinic dehydrogenase
4. Cytochrome oxidase

Q.

1. Apoenzyme - Non - Protein
   Co - factor - Protein
2. Apoenzyme - Protein
   Co - factor - Non-Protein
3. Apoenzyme - Protein
   Co - factor - Protein
4. None of the above
   

Q. Enzyme pepsin belongs to category

1. oxidoreductases
2. transferases
3. hydrolases

4. isomerases

Q. Most of the digestive enzymes belong to the class of ________.

1. Lyases
2. Hydrolases
3. Oxidoreductases
4. Transferases.

Q. Which one of the following hydrolyzes internal phosphodiester bonds in a polynucleotide chain:

1. Lipase
2. Protease
3. Exonuclease
4. Endonuclease

Q. Enzyme pepsin belongs to category

1. oxidoreductases
2. transferases
3. hydrolases

4. isomerases

Q. How do proteins differ from carbohydrates and lipids?

Q. Phosphorylation of glucose during glycolysis is catalysed is

1. Phosphoglucomutase
2. Phosphoglucoisomerase
3. Hexokinase
4. Phosphorylase

Q.

1. Substrate concentration at which the rate of reaction is maximum.
2. The rate of reaction
3. The rate of enzymatic activity
4. Substrate concentration at which the reaction attains half of its maximum velocity

Q. Enzymes catalysing the breakdown of larger molecules into smaller molecules are

1. hydrolases
2. isomerases
3. ligases
4. both (a) and (c)

Q. Enzyme pepsin belongs to category

1. oxidoreductases
2. transferases
3. hydrolases

4. isomerases

Q. Which one of the following coenzymes is required for the conversion of L-alanine to a racemic mixture of D- and L-alanine?

1. Thiamine pyrophosphate
2. Flavin adenine dinucleotide
3. Coenzyme A
4. Pyridoxal-$6$-phosphate

Q. Which one of the following hydrolyzes internal phosphodiester bonds in a polynucleotide chain:

1. Lipase
2. Protease
3. Exonuclease
4. Endonuclease