Insertion Mutation

Q. Under which of the following conditions there will be no change in the reading frame of following mRNA?
5' AACAGCGGUGCUAUU 3'

1. Deletion of GGU from 7th, 8th and 9th positions
2. Insertion of G at 5th position
3. Deletion of G from 5th position
4. Insertion of A and G at 4th and 5th position respectively

Q.

Point mutation involves

1. Insertion

2. Deletion

3. Duplication

4. Change in single base pair

Q. Which of the following statement is not correct about a cloning vector ?

1. It is generally small in size.
2. ‘Ori’ is a sequence responsible for controlling the copy number of the linked DNA.
3. Selectable marker selectively permitting the growth of the non-transformants.
4. In order to link the alien DNA, the vector needs to have single recognition site for the commonly used restriction enzymes.

Q. A frameshift mutation will have minimum effect when it leads to ______________

1. insertion of 2 bases
2. deletion of 1 base
3. deletion of 3 bases
4. deletion of 2 bases

Q. The normal sequence of mRNA transcribed from a particular DNA strand is
5'-AUG GUC GGA CUG GGC GUC UAA-3'.
As a result of mutation in DNA, the mRNA sequence is changed to
5'-AUG GUC UGA CUG GGC GUC UAA-3'.
What type of mutation is this?

1. silent
2. missense
3. nonsense
4. frameshift

Q.

What do you mean by DNA polymorphism in simple words?

Q. Which enzyme/s will be produced in a cell in which there is a nonsense mutation in the lac $y$ gene?

1. Lactose permease
2. $\beta$-galactosidase
3. Transacetylase
4. Lactose permease and transacetylase

Q. Given in the figure is chromosomal mutation. It is

1. Duplication
2. Reciprocal translocation
3. Deletion
4. Inversion

Q. The insertion or deletion of a base pair into the genetic code will cause a frameshift mutation unless the number of base pairs inserted or deleted is

1. 1
2. 2
3. 3
4. 10

Q. An mRNA is transcribed from a $DNA$ segment having the base sequence $3\text{'}CTCGGC$ $AATCCG5\text{'}$. What will be the correct order of binding of the four aminoacyl-tRNA complexes given below during translation of this mRNA?

1. $P,Q,R,S$
2. $R,P,S,Q$
3. $Q,P,R,S$
4. $S,Q,P,R$

Q. A non-sense mutation results into

1. Change in protein structure
2. Stoppage of protein synthesis
3. Termination of polypeptide chain
4. Stoppage of transcription

Q. Select the option which correctly tells the position of components in the transcription unit.

1. Terminator – located towards 5’ end of coding strand of gene

2. Operator - located towards 3’ end of coding strand of gene
3. Promoter – located towards 3’ end of coding strand of gene
4. Promoter – located towards 5’ end of coding strand of gene

Q. Suppose the correct sequence of nucleotides on a single DNA strand is AGGCTGTTAT. A mutation occurs, and the sequence becomes AGGCATGTTAT. Which of the following types of mutation is this?

1. Deletion
2. Insertion
3. Base substitution
4. Non-frameshift mutation

Q. The mutation of a purine to a pyramidine is known as

1. Transition
2. Frame shift
3. Transversion
4. Non sense

Q. When a mutation is limited to the substitution of one nucleotide for another, it is called _________.

1. Translocation
2. Point mutation
3. Base inversion
4. Sugar phosphate deletion
5. Frameshift mutation

Q. If in a DNA, codons are ATGATGATG and cytosine base is inserted at the beginning, which of the following would be the result?

1. A non-sense mutation
2. CA, TGA, TGA, TG
3. CAT, GAT, GAT, G
4. C, ATG, ATG, ATG

Q. To be evolutionary successful, the mutation must occur in

1. Germplasm
2. Somatoplasm
3. Karyolymph/zygote
4. Ergastoplasm

Q. Suppose the correct sequence of nucleotides on a single DNA strand is AGGCTGTTAT. A mutation occurs, and the sequence becomes AGGCATGTTAT. Which of the following types of mutation is this?

1. Deletion
2. Insertion
3. Base substitution
4. Non-frameshift mutation

Q. Which of the following is most effective chemical mutagen?

1. Methane
2. Guanine
3. N-ethyl-N-nitrosourea
   
4. Caffeine