RNA Polymerase

Q. In eukaryotes, RNA polymerase III is responsible for synthesis of:

1. 28$S$ rRNA, 18$S$ rRNA and 5.8$S$ rRNA
2. $t$RNA, $hn$RNA, $r$RNA
3. $t$RNA, 5$S$ rRNA, $sn$RNA
4. $hn$RNA, $t$RNA, $r$RNA

Q. Consider the following statements
1. The promoter is located towards the 5’ end of the structural gene with respect to the template strand.
2. The promoter is located towards the 3’ end of the structural gene with respect to the template strand.
3. The terminator is located towards the 3’ end of the structural gene with respect to the template strand.
4. The terminator is located towards the 5’ end of the structural gene with respect to the template strand.

The correct statements are:

1. 1 and 2
2. 2 and 3
3. 3 and 4
4. 2 and 4

Q. Explain the process of making heterogenous nuclear RNA (hnRNA) into a fully functional mRNA in eukaryotes. Where does this process occur in the cell? [3 Marks]

Q. During transcription, mRNA provide A, tRNA brings B and reads genetic code and rRNAs play C and catalytic role during D. Choose the correct option for A, B, C and D.

1. A-template, B-amino acid, C-structural, D-translation
2. A-template, B-genetic codes, C-structural, D-translation
3. A-template, B-genetic codes, C-translation, D-structural
4. A-template, B-amino acid, C-translation, D-structural

Q. The following nucleotides is used for elongation of RNA chain by RNA polymerase except

1. GTP
2. TTP
3. ATP
4. UTP

Q. Consider the following statements. In eukaryotes
I. RNA polymerase I transcribes rRNAs
II. RNA polymerase II transcribes snRNAs
III. RNA polymerase III transcribes hnRNA
IV. RNA polymerase II transcribes hnRNA
Which of the statements given above are correct?

1. I and II
2. I and III
3. II and III
4. I and IV
5. I, II and IV

Q. Consider the following statements.
I. rRNA provides the template for synthesis of proteins.
II. tRNA brings amino acids and reads the genetic code.
III. RNA polymerase binds to promoter and initiates transcription.
IV. A segment of DNA coding for polypeptide is called intron.
Which of the statements given above are correct?

1. I and III
2. I and II
3. I, II and III
4. II and III

Q. Why does hnRNA undergo tailing and capping?

Q.

What are the two functions of introns?

Q.

What is the number of RNA polymerases found in eukaryotes?

Q. How many types of RNA polymerases are present in bacteria?

1. Five
2. Three
3. One
4. Two

Q.

hnRNA is formed by which enzyme?

Q. Assertion: Template or antisense strand, having 3'→5' polarity takes part in transcription.
Reason: Nontemplate or sense strand, having 5'→3' polarity, does not take part in transcription.

1. Both assertion and reason are true and reason is the correct explanation of assertion
2. Both assertion and reason are true but reason is not the correct explanation of assertion
3. Assertion is true but reason is false
4. Both assertion and reason are false

Q. During transcription, RNA polymerase holoenzyme binds to a gene promoter and assumes a saddle-like structure. What is its DNA-binding sequence?

1. CACC
2. TATA
3. TTAA
4. AATT

Q. What is the difference between restriction endonuclease type 1 and type 3?

Q. In eukaryotes, which enzyme catalyzes the formation of 5S rRNA?

1. RNA polymerase – I
2. DNA polymerase –I
3. RNA polymerase – II
4. RNA polymerase – III

Q.

Why only one strand of DNA is involved in mRNA formation ?

Q. In eukaryotes mRNA is synthesised with the aid of

1. RNA polymerase III
2. RNA polymerase II
3. RNA polymerase I
4. Reverse transcriptase

Q.

name the region of the mRNA that is not translated what is its role

Q. RNA polymerase stops its activity by

1. sigma $\left(\sigma \right)$ factor
2. rho $\left(\rho \right)$ factor
3. UAG
4. AUG

Q. In prokaryotes, holoenzyme RNA polymerase attaches to the DNA sequence at the promoter site of template DNA during transcription. The sequence of that point where the RNA polymerase binds is:

1. AATT
2. TTAA
3. TATA
4. CACC

Q.

The enzyme required for transcription is:

1. RNAase

2. Endonuclease

3. RNA polymerase

4. DNA polymerase

Q.

During transcription, the DNA site at which RNA polymerase binds is called

1. Enhancer

2. Regulator

3. Promoter

4. Receptor

Q.

Do restriction enzymes exist in eukaryotes?

Q. Code transfer for synthesis of polypeptide involves

1. DNA, tRNA, rRNA and mRNA
2. tRNA, DNA, mRNA and rRNA
3. mRNA, tRNA, rRNA and DNA
4. DNA, mRNA, tRNA and amino acids.

Q.

The element responsible for the ring structure of chlorophyll and maintenance of ribosome structure is

1. $M{g}^{+}$

2. $K^{+}$

3. $C{a}^{++}$

4. $S$

Q. If the base sequence of one DNA template strand is CAACATGTAGCAAGT, what would be the base sequence in the RNA transcribed against it?

1. GTTGTACATCGTTCA
2. CUUCUAGAUGCUUGU
3. GUUGUACAUCGUUCA
4. CAACAUGUAGCAAGU

Q. What is vat dye?

Q. What is a cistron? [1]

Q. What is negative gene regulation?