Recombinant DNA Technology

Q.

What is meant by Eastern blotting and western blotting South blotting Northern blotting and please don't copy paste the explanation given in Wikipedia

Q.

Purpose of agarose gel electrophoresis is to _____________.

1. cut DNA into many segments.
2. separate DNA fragments on the basis of their molecular weight.
3. construct rDNA by joining DNA with cloning vectors
4. isolation of DNA molecule

Q. Regarding assertion and reason, select the correct option

Assertion: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote)
Reason: Both bacteria and yeast multiply very fast to form huge population which express the desired gene.

1. Both Assertion and Reason are true and Reason is correct explanation of the Assertion.
2. Both Assertion and Reason are true but Reason is not the correct explanation of Assertion
3. Assertion is true but the Reason is false
4. Both Assertion and Reason are false

Q.

Can you suggest a method to remove oil (hydrocarbon) from seeds based on your understanding of rDNA technology and chemistry of oil?

Q.

What is gene splicing used for?

Q. The gene product of Lac A gene in lac operon is

1. Beta-galactoside permease

2. Beta-galactoside transacetylase

3. Beta-galactosidase

4. Beta-galactoside isomerase

Q.

Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutic (use the internet).

Q.

Two microbes found to be very useful in genetic engineering are

1. Vibrio cholerae and a tailed bacteriophage

2. Diplococcus sp. and Pseudomonas sp.

3. Crown gall bacterium and Caenorhabditis elegans

4. Escherichia coli and Agrobacterium tumefaciens

Q. Linking of antibiotic resistance gene with the plasmid vector is brought about by

1. DNA polymerase
2. RNA polymerase
3. DNA ligase
4. restriction enzymes

Q. ____________ of mRNA takes place during the process of RNAi.

1. Translation
2. Silencing
3. Transcription
4. Splicing

Q. Match column I with their correct match in column II.

The correct match is

1. l-A, II-D, III-B, IV- C
2. l-C, II-A, III-B, IV- D
3. l-D, II-B, III-C, IV- A
4. l-B, II-A, III-D, IV- C

Q. The construction of the first recombinant DNA was done by using the native plasmid of

1. Escherichia coli
2. Salmonella typhimurium
3. Bacillus thuringiensis
4. Yeast
5. Agrobacterium

Q. Give a brief account of the tools of recombinant DNA technology.

Q. Recombinant DNA, that contains our gene of interest is also called __ DNA.

Q. Mention the complete steps involved in RNA interference (RNAi) in Biotechnology in an easy language.

Q. Which of the following is not a property of a plasmid?

1. Autonomous replication
2. Ori site
3. Single-stranded
4. Double-stranded

Q. _________ genes are the one which directly control the switching off and on of the transcription process, in the operon model of gene regulation.

1. Operator
2. Promotor
3. Regulatory
4. Structural

Q. Which of the following tools are used in recombinant DNA technology?

1. Plasmids
2. DNA ligase
3. Restriction enzymes
4. All of these

Q. More than One Answer Type
एक से अधिक उत्तर प्रकार के प्रश्न

Study the figure below and choose the correct statement(s) w.r.t. gel electrophoresis.

जेल इलेक्ट्रोफोरेसिस के संदर्भ में नीचे दिए गए चित्र का अध्ययन कर सही कथन/कथनों का चयन कीजिए।

1. Agarose is used as a matrix, which is a natural polymer extracted from marine fishes.
   
   ऐगारोज का उपयोग आधात्री के रूप में किया जाता है, जो समुद्री मछलियों से निकाला गया एक प्राकृतिक बहुलक है
2. DNA fragments separate according to their size through sieving effect provided by agarose gel.
   
   DNA खंडों को ऐगारोज जेल के छलनी प्रभाव द्वारा उनके आकार के अनुसार अलग करते हैं
3. DNA fragments move from anode to cathode under an electric field through agarose matrix.
   
   DNA खंड, ऐगारोज आधात्री के माध्यम से विद्युत क्षेत्र में ऐनोड से कैथोड की ओर गति करते हैं
4. The separated DNA fragments can be visualised after staining them with ethidium bromide under UV light.
   
   पृथक्कृत DNA खंडों को पराबैंगनी प्रकाश में इथीडियम ब्रोमाइड से अभिरंजित करने के पश्चात् देखा जा सकता है

Q. The portion of the eukaryotic genes which are not a part of mature mRNA:

1. Introns
2. Exons
3. Codons
4. Cistrons

Q. Autonomously replicating, circular, extra-chromosomal DNA used for construction of first recombinant DNA was isolated from

प्रथम पुनर्योगज DNA के निर्माण के लिए प्रयुक्त स्वतः प्रतिकृति करने वाले वृत्ताकार, गुणसूत्र बाह्य DNA को किससे पृथक किया गया था?

1. Bacillus thuringiensis
   
   बैसिलस थुरीनजिऐंसिस
2. Salmonella typhimurium
   
   साल्मोनेला टाइफीमूरियम
3. Escherichia coli
   
   एशरिकिआ कोलाई
4. Agrobacterium tumefaciens
   
   एग्रोबैक्टीरियम ट्यूमीफेशियन्स

Q. In order to calculate map distance of genes on a chromosome, what must we know?

1. Cross over percentage
2. Recombination frequency of each gene locus
3. Non-cross over percentage
4. Number of mutant genes

Q. DNA ligase is an enzyme that catalyses the

1. Splitting of DNA threads into small bits
2. Joining of the fragments of DNA
3. Denaturation of DNA
4. Synthesis of DNA

Q. Plasmids found in bacteria are:

1. Extranuclear DNA
2. Food particles
3. Dead protoplasmic parts
4. None of the above

Q. Which of the following represents the correct sequence of steps in recombinant DNA technology?

I. Isolation of the DNA fragment or genes
II. Introduction of rDNA into the host
III. Expression of the introduced gene in the host
IV. Selection and identification of the transformed host
V. Insertion of the isolated gene in a suitable vector

1. I→ III → II → IV → V
2. III → II → I → V → IV
3. I → V → II → IV → III
4. V → I → III → IV → II

Q. With regards to DNA extraction which of the following is/are correct?

1. Only the purest DNA samples can be used in rDNA technology.
2. DNA is present inside the nucleus of the cell which is embedded in the cytoplasm along with many organelles.
3. DNA extraction increases the success of recombination.
4. Since DNA extraction is easy, the step need not be avoided.

Q. Distance between the genes and the percentage of recombination shows

1. A direct relationship
2. A parallel relationship
3. An inverse relationship
4. No relationship

Q. Genetic engineering has been successfully used for producing:

1. Transgenic models for studying new treatment for certain cardiac diseases
2. Transgenic Cow–Rosie which produces high-fat milk for making ghee
3. Animals like bulls for farm work as they have super power
4. Transgenic mice for testing safety of polio vaccine before using in humans

Q. All of the following are required during DNA synthesis on lagging strand, except

निम्नलिखित में से किसके अतिरिक्त सभी की आवश्यकता लैगिंग रज्जुक पर डीएनए (DNA) संश्लेषण के दौरान होती है?

1. DNA ligase
   
   डीएनए (DNA) लाइगेज
2. DNA polymerase
   
   डीएनए (DNA) पॉलिमरेज
3. DNA dependent RNA polymerase
   
   डीएनए (DNA) आश्रित आरएनए (RNA) पॉलिमरेज
4. DNase
   
   डीएनएज (DNase)

Q.

Whatis DNA fingerprinting? Mention its application.