Reverse Transcriptase

Q.

Retroviruses have genetic material

1. Either DNA or RNA only

2. DNA or RNA only

3. DNA only

4. RNA only

Q. In the biochemical characterisation of the transforming principle, it was found that the process of transformation is not affected by which of the following enzymes?
A. DNase
B. RNase
C. Peptidase
D. Lipase

1. A, B, D
2. A, C, D
3. B, C, D
4. A, B, C

Q. Consider the following statements (A-D) and select the option stating which ones are true (T) and false (F).
A. The first clinical gene therapy was carried out in 1991.
B. A short single stranded DNA or RNA tagged with a radioactive molecule to detect the complementary strand is called a probe.
C. The amplification technique used for obtaining multiple copies of specific sequences of DNA is called PCR.
D. The mature proinsulin contains A and B polypeptides linked together by disulphide bonds.

1. A - F, B - T, C - T, D - F
2. A - F, B - T, C - F, D - T
3. A - F, B - F, C - T, D - F
4. A - F, B - T, C - F, D - F

Q. Which of the following is responsible for adding nucleotides to the growing chain of DNA during replication?

1. Ligase
2. DNA polymerase
3. Topoisomerase
4. Both a and b

Q. Which enzyme present in retroviruses converts single stranded RNA into a double stranded viral DNA?

1. RNA polymerase
2. Transcriptase
3. Reverse transcriptase
4. DNA polymerase

Q. DNA can be formed over RNA through

1. Ligase
2. Gyrase
3. Helicase
4. Reverse transcriptase

Q. cDNA is formed using the mRNA as the template. Since RNA to DNA replication does not normally occur in nature, the enzyme ________ is employed to obtain cDNA from mRNA.

1. Reverse transcriptase
2. Circular DNA
3. Recombinant DNA
4. Cloning

Q. Which of the following options correctly explains the sequence of processes in PCR?

1. 
   Primer annealing → DNA synthesis → Denaturation
2. 
   Primer annealing → Denaturation → DNA synthesis
3. 
   Denaturation → Primer annealing → DNA synthesis
4. 
   Denaturation → DNA synthesis → Primer annealing

Q. Who discovered synthesis of DNA from RNA in rous sarcoma virus?

1. Smith
2. Twort
3. Temin
4. Meyer

Q. How is the wrong base removed before proceeding to add new bases in the 5-3 direction during DNA replication?

Q. The information from RNA to DNA are transferred by the process, which is called as

1. Replication
2. Transcription
3. Translation
4. Reverse transcription

Q. Retroviruses are unique because they perform

1. Reverse replication
2. Reverse transcription
3. Reverse translation
4. Reverse splicing

Q. DNA fragments result when.......... cut DNA molecules at specific sites.

1. RFLPs
2. DNA probes
3. restriction enzymes
4. DNA polymerase

Q. The reverse transcriptase is used by

1. The plasmids of competent cells
2. Retrovisuses
3. Purple sulfur bacteria
4. Plasmodia
5. Archaebacteria

Q. Any sort of damage to DNA is detected in the

1. S phase
2. M phase
3. G2 phase
4. All of the above

Q. Assertion :Retroviruses or transposons can be used in the process of RNAi. Reason: RNAi takes place in all eukaryotic organisms as a method of cellular defense.

1. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
2. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
3. Assertion is correct but Reason is incorrect
4. Both Assertion and Reason are incorrect

Q. The enzyme reverse transcriptase is

1. RNA dependent RNA polymerase
2. DNA dependent DNA polymerase
3. DNA dependent RNA polymerase
4. RNA dependent DNA Polymerase

Q. Which of the following is responsible for adding nucleotides to the growing chain of DNA during replication?

1. Ligase
2. DNA polymerase
3. Topoisomerase
4. Both a and b

Q. DNA is transcribed by some viral RNA using the enzyme

1. DNA polymerase
2. Reverse transcriptase
3. Ligase
4. Endonuclease

Q. cDNA is formed using the mRNA as the template. Since RNA to DNA replication does not normally occur in nature, the enzyme ________ is employed to obtain cDNA from mRNA.

1. Reverse transcriptase
2. Cloning
3. Circular DNA
4. Recombinant DNA

Q.

in retroviruses the flow is in opposite direction i.e

RNA----> DNA----->RNA------> PROTIENS.

then why is rna converted into dna if the protien is synthesised from RNA?

Q. Genetic material of retroviruses is

1. DNA
2. RNA
3. DNA and RNA
4. Nucleoprotein complexes

Q.

what can be a simple name for the process in which genetic material flows from RNA TO DNA?

Q. Central dogma of genetic information is modified by the discovery of which of the following enzymes?

1. DNA polymerase
2. Reverse transcriptase
3. RNA polymerase
4. Ligase

Q. Methylation of DNA most commonly occurs in which of the following sequence?

1. $C_{p}G$
2. $C_{p}A$
3. $C_{p}T$
4. $C_{p}C$

Q. Reverse transcriptase is the enzyme which makes from
an template.

1. DNA
2. RNA
3. cDNA
4. rDNA

Q.

Which of the following is true about reverse transcriptase?

1. It uses mRNA as a template

2. The central dogma of life is violated

3. It is found only in certain viruses
   

4. All of the above

Q. Reverse transcriptase is the enzyme which makes

1. DNA from RNA template
2. DNA from DNA template
3. RNA from DNA template
4. RNA from RNA template

Q. Reverse transcription was discovered by which of the following scientist?

1. Watson and Crick
2. Meischer
3. Temin and Baltimore
4. Khorana

Q. Temin worked on virus

1. Rhinovirus
2. Herpes virus
3. Retrovirus
4. Dengue virus